A Certificate of Analysis (COA) contains valuable information about a product, ingredient, environmental sample, or food testing result.

However, many organizations receive laboratory reports and immediately ask: What does this result actually mean?

A Certificate of Analysis should do more than provide numbers. It should help support decisions regarding:

  • Product quality
  • Food safety
  • Supplier approval
  • Product release
  • Shelf-life evaluation
  • Regulatory requirements
  • Customer specifications

Understanding how to read a COA helps manufacturers, processors, quality assurance teams, and purchasing departments use laboratory data effectively.

PBR Laboratories helps clients understand results and make informed decisions—not simply receive data.

WHAT IS A CERTIFICATE OF ANALYSIS (COA)?

A Certificate of Analysis is a laboratory report that documents the results obtained from testing a submitted sample.

Depending on the testing performed, a COA may include:

Sample Information
Test Methods
Analytical Results
Units of Measurement
Detection Limits
Comments or Notes
Reporting Dates
Laboratory Identification Information

The COA becomes part of the quality documentation used to support product and process decisions.

THE MOST IMPORTANT SECTIONS OF A COA

Sample Information

The first section typically identifies:

Sample Name | Product Description | Sample Identification Number | Client Reference Information | Date Received | Date Reported

Before reviewing results, confirm the correct sample was tested.

Test Parameters

This section identifies what was tested. Examples may include:

Aerobic Plate Count (APC) | Yeast & Mold | Total Coliforms | Escherichia coli (E. coli) | Salmonella spp. | Listeria monocytogenes | Water Activity (aw) | Moisture Content | Nutritional Parameters

Always verify that the requested testing appears on the report.

Understanding Result Values

Results may be reported in different formats depending on the test.

Colony Forming Units per Gram (CFU/g)

Commonly used for food microbiology testing. Examples: <10 CFU/g, 100 CFU/g, 1,000 CFU/g, 10,000 CFU/g. Used for APC, Yeast & Mold, Total Coliforms, Enterobacteriaceae, and LAB.

Colony Forming Units per Millilitre (CFU/mL)

Often used for liquids. Examples: Water samples, dairy products, beverages.

Percentage (%)

Used for: Moisture Content, protein, fat, ash, fibre, and other nutritional parameters.

Detected / Not Detected

Commonly used for pathogen testing. Examples: Salmonella spp., Listeria monocytogenes, E. coli O157:H7, STEC.

RESULT INTERPRETATIONS

What Does "<10 CFU/g" Mean?

This is one of the most common questions. A result reported as <10 CFU/g means the organism was not detected above the method's reporting limit. It does not necessarily mean: Zero Organisms Are Present. It means the level was below the reporting threshold of the test method.

What Does "Detected" Mean?

A Detected result indicates the target organism was identified in the sample. Examples: Salmonella spp. – Detected, Listeria monocytogenes – Detected, E. coli O157:H7 – Detected. The significance of the result depends on: product type, sample type, intended use, customer requirements, and food safety objectives.

What Does "Not Detected" Mean?

A Not Detected result indicates the target organism was not identified under the conditions of the test. Examples: Salmonella spp. – Not Detected, Listeria monocytogenes – Not Detected, E. coli O157:H7 – Not Detected. Results should always be interpreted within the context of the testing performed.

Understanding Detection Limits

Every analytical method has a detection capability. A laboratory may report: Less Than (<), Not Detected, or Below Reporting Limit. These terms indicate the result was below the method's reporting threshold. Understanding detection limits helps prevent misinterpretation.

WHAT SHOULD YOU LOOK FOR FIRST?

When reviewing a COA:

1
Step 1 – Confirm Sample Information

Ensure the correct sample was tested.

2
Step 2 – Review Requested Parameters

Verify all requested tests were completed.

3
Step 3 – Review Results

Identify any unexpected findings.

4
Step 4 – Compare To Specifications

Evaluate results against: Internal specifications | Customer requirements | Historical data | Program objectives

5
Step 5 – Evaluate Trends

Historical trends often provide more value than a single result.

COMMON RESULTS FOUND ON COAs

Microbiology Results
  • Aerobic Plate Count (APC): Overall microbial populations.
  • Yeast & Mold: Spoilage potential & shelf-life performance.
  • Total Coliforms: Hygiene and sanitation conditions.
  • Escherichia coli (E. coli): Additional hygiene indicators.
  • Enterobacteriaceae: Sanitation and process control.
  • Food Pathogens: Salmonella spp., Listeria monocytogenes, E. coli O157:H7.
Quality & Chemistry Results
  • Water Activity (aw): Microbial growth potential.
  • Moisture Content: Formulation consistency.
  • Nutritional Parameters: Protein, fat, fibre, ash, carbohydrates.
  • Shelf-Life Testing Results: Product stability over time.

COMMON MISTAKES WHEN READING A COA

Looking At Only One Result

Results should be interpreted within the context of the entire report.

Ignoring Historical Data

Trend analysis often provides the most valuable insight.

Assuming "Not Detected" Means Zero

Detection limits always matter.

Ignoring Sample Information

Incorrect sample identification can lead to incorrect decisions.

Treating Every Result The Same

Interpretation depends on product type, specifications, and objectives.

WHAT DECISIONS DOES A CERTIFICATE OF ANALYSIS SUPPORT?

Product Release Decisions
Supplier Verification
Shelf-Life Validation
Food Safety Programs
Product Quality Programs
Customer Specification Verification
Corrective Action Investigations

WHY UNDERSTANDING A COA MATTERS

Laboratory testing generates data. A Certificate of Analysis helps transform that data into decisions.

The most effective use of a COA is not simply reviewing numbers. It is understanding:

  • What was tested
  • Why it was tested
  • What the results mean
  • What actions should happen next

That approach helps organizations improve food safety, product quality, and operational performance.

Frequently Asked Questions

A laboratory report documenting analytical results for a submitted sample.

Colony Forming Units per Gram, a common reporting unit used in food microbiology testing.

The organism was not detected above the reporting limit of the test method.

The target organism was identified in the sample.

The target organism was not identified under the conditions of the test.

Trend analysis often provides more useful information than a single result.

Need Help Understanding Laboratory Results?

PBR Laboratories provides food microbiology testing, food pathogen testing, nutritional analysis, shelf-life studies, food quality testing, and environmental monitoring services throughout Alberta, Western Canada, and Canada.

If you have questions about a Certificate of Analysis, microbiology results, pathogen testing results, or food quality data, contact PBR for decision-focused laboratory support.

Contact PBR Laboratories

Choose PBR – Because Precision Matters, Defining Excellence in Laboratory Services Since 1984.