Lactic Acid Bacteria (LAB) can be either beneficial or problematic depending on the product being tested.

In some foods, Lactic Acid Bacteria are intentionally added to create desired characteristics.

In other foods, elevated LAB counts may indicate spoilage, shelf-life limitations, or process control issues.

Understanding the context of the result is critical. Questions manufacturers often ask include:

  • Is this result normal?
  • Is the product fermenting?
  • Does this affect shelf life?
  • Should corrective action be taken?

PBR Laboratories provides Lactic Acid Bacteria testing, food microbiology testing, dairy testing, and shelf-life studies throughout Alberta, Western Canada, and Canada.

WHAT ARE LACTIC ACID BACTERIA?

Lactic Acid Bacteria are a group of microorganisms that produce lactic acid as they metabolize sugars.

Common groups include:

Lactobacillus spp.
Lactococcus spp.
Leuconostoc spp.
Pediococcus spp.
Streptococcus thermophilus

These organisms are naturally found in many food products and processing environments.

WHY ARE LACTIC ACID BACTERIA IMPORTANT?

Lactic Acid Bacteria play a major role in:

Fermentation

Production of yogurt, cheese, cultured dairy products, fermented meats, and fermented foods.

Product Preservation

Acid production may help inhibit growth of undesirable microorganisms.

Shelf Life

LAB can either extend shelf life or contribute to spoilage depending on the product.

Product Quality

LAB influence flavor, aroma, texture, and product stability.

HOW ARE LACTIC ACID BACTERIA RESULTS REPORTED?

Results are typically reported as:

Colony Forming Units per Gram (CFU/g)

Examples:
<10 CFU/g
100 CFU/g
10,000 CFU/g
1,000,000 CFU/g

Colony Forming Units per Millilitre (CFU/mL)

Examples:
<10 CFU/mL
100 CFU/mL
100,000 CFU/mL

Interpretation depends entirely on the product and intended use.

WHEN ARE HIGH LAB COUNTS A GOOD THING?

In fermented foods, elevated LAB counts are often expected.

Examples include:

Yogurt
Cheese
Kefir
Fermented Dairy Products
Fermented Sausages
Cultured Foods

In these products, LAB contribute to:

  • Flavor development
  • Texture formation
  • Product stability
  • Fermentation performance

WHEN ARE HIGH LAB COUNTS A PROBLEM?

In non-fermented products, elevated LAB counts may indicate:

Product Spoilage
Shelf-Life Limitations
Temperature Abuse
Process Control Issues
Packaging Problems
Extended Storage Conditions

COMMON SIGNS OF LAB-RELATED SPOILAGE

Sour Flavors
Increased Acidity
Gas Production
Package Swelling
Texture Changes
Product Separation
Reduced Shelf Life

PRODUCTS COMMONLY TESTED FOR LAB

Dairy Products

One of the most common applications. Examples: Milk | Cheese | Yogurt | Cultured dairy products

Fermented Foods

Evaluate fermentation performance and consistency.

Ready-to-Eat Foods

Investigate spoilage concerns.

Processed Foods

Monitor product stability and quality.

Shelf-Life Studies

Track microbiological changes over time.

WHAT DOES A HIGH LAB RESULT MEAN?

The answer depends on the product.

Scenario 1 – Yogurt Product

High LAB count may indicate: Expected fermentation activity | Active culture performance | Product consistency

Scenario 2 – Shelf-Stable Food

High LAB count may indicate: Spoilage development | Reduced shelf life | Process control concerns

Scenario 3 – Refrigerated Product

High LAB count may indicate: Product aging | Storage impacts | Shelf-life limitations

WHAT FACTORS INFLUENCE LAB GROWTH?

Temperature

Improper storage may accelerate growth.

Product Formulation

Sugar content and nutrients influence growth.

Water Activity (aw)

Available water supports microbial activity.

Packaging Conditions

Package atmosphere may affect growth rates.

Storage Time

LAB populations may increase throughout shelf life.

LAB AND SHELF-LIFE TESTING

Lactic Acid Bacteria are frequently included in shelf-life studies because they may help explain:

Flavor Changes
Acidity Changes
Product Stability
Texture Changes
Product Spoilage

Tracking LAB over time helps manufacturers understand how products behave throughout their intended shelf life.

WHAT SHOULD YOU DO AFTER A HIGH LAB RESULT?

1
Step 1 – Consider Product Type

Determine whether elevated LAB are expected.

2
Step 2 – Review Historical Results

Compare current results to previous production lots.

3
Step 3 – Evaluate Shelf-Life Data

Assess product age and storage conditions.

4
Step 4 – Review Process Controls

Evaluate production conditions and formulation consistency.

5
Step 5 – Consider Additional Testing

Additional testing may include: Aerobic Plate Count (APC) | Yeast & Mold | Total Coliforms | Water Activity (aw) | Shelf-Life Studies

LAB COMPARISONS

LAB vs Aerobic Plate Count (APC)

Aerobic Plate Count (APC): Measures total aerobic microorganisms.

Lactic Acid Bacteria (LAB): Measures a specific group of microorganisms.

Both tests provide useful but different information.

LAB vs Yeast & Mold

Lactic Acid Bacteria: Often associated with fermentation and acid production.

Yeast & Mold: Often associated with spoilage, visible growth, and shelf-life concerns.

These tests are frequently reviewed together during shelf-life investigations.

WHAT DECISIONS DOES LAB TESTING SUPPORT?

Shelf-Life Validation
Dairy Quality Programs
Fermentation Monitoring
Product Development
Product Stability Studies
Food Quality Investigations

COMMON MISCONCEPTIONS

“High LAB Counts Always Mean Spoilage”

False. In fermented products, elevated LAB counts may be expected and desirable.

“LAB Are Pathogens”

False. LAB are generally not considered foodborne pathogens.

“LAB Testing Is Only For Dairy Products”

False. LAB testing is used across many food categories.

“LAB Results Should Be Evaluated Alone”

False. Results should be reviewed alongside other microbiological and quality parameters.

WHY LAB TESTING MATTERS

Lactic Acid Bacteria testing helps manufacturers:

  • Evaluate fermentation performance
  • Monitor shelf life
  • Investigate spoilage
  • Improve product consistency
  • Support dairy quality programs
  • Understand microbiological changes over time

The goal is not simply generating laboratory results.

The value of testing comes from understanding whether LAB activity is beneficial or problematic within the specific product.

FAQ

What are Lactic Acid Bacteria?
A group of microorganisms that produce lactic acid during growth and fermentation.
Are Lactic Acid Bacteria harmful?
Generally no. They are widely used in food production and fermentation.
Why are LAB tested?
To monitor fermentation, shelf life, spoilage, and product quality.
How are results reported?
Typically as Colony Forming Units per Gram (CFU/g) or Colony Forming Units per Millilitre (CFU/mL).
Do high LAB counts always indicate spoilage?
No. In fermented foods, elevated counts may be expected.
What products commonly require LAB testing?
Dairy products, fermented foods, ready-to-eat foods, processed foods, and shelf-life studies.

Need Help Understanding Lactic Acid Bacteria Results?

PBR Laboratories provides Lactic Acid Bacteria testing, food microbiology testing, dairy testing, shelf-life studies, water activity testing, and food quality testing services throughout Alberta, Western Canada, and Canada.

Contact PBR to discuss microbiological results, fermentation monitoring, shelf-life objectives, product stability concerns, and food quality programs.

Contact PBR Laboratories

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